5K7E

The structure of pistol ribozyme, soaked with Mn2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-22
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.7712
Spacegroup name	P 32 2 1
Unit cell lengths	55.986, 55.986, 89.460
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.500 - 3.270
Rwork	0.240
R-free	0.29000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5k7d
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	89.500	3.530
High resolution limit [Å]	3.270	3.270
Rpim		0.589
Number of reflections	3743
<I/σ(I)>	9.4	1.1
Completeness [%]	95.7
Redundancy	3.1	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	containing 0.1 M Na-cacodylate, pH 6.5, 0.05 M Mg(OAc)2 and 32% MPD and supplemented with 150 mM MnCl2