5K6W

Sidekick-1 immunoglobulin domains 1-5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4C
Synchrotron site	NSLS
Beamline	X4C
Temperature [K]	100
Detector technology	CCD
Collection date	2013-05-04
Detector	MAR CCD 165 mm
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	70.140, 152.870, 158.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.997 - 3.500
R-factor	0.2044
Rwork	0.202
R-free	0.25100
RMSD bond length	0.003
RMSD bond angle	0.613
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.670
High resolution limit [Å]	3.500	3.500
Rmerge	0.132	0.304
Number of reflections	19853
<I/σ(I)>	9.9	3.9
Completeness [%]	89.9	79.5
Redundancy	3.3	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		295	1.4M ammonium sulfate, 0.5M lithium chloride, 10mM yttrium chloride