5K6Q
Crystal structure of Arabidopsis thaliana acetohydroxyacid synthase catalytic subunit
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-09-25 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.95370 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 178.934, 178.934, 186.086 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.557 - 2.952 |
| R-factor | 0.1926 |
| Rwork | 0.191 |
| R-free | 0.22910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yhy |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.603 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.560 | 3.000 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.101 | 0.907 |
| Number of reflections | 37387 | |
| <I/σ(I)> | 31.3 | 2.1 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 15.8 | 13.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.8 | 290 | Na/K tartrate, lithium sulfate, CHES, FAD, ThDP, MgCl2 |
