5K6N
Structure of a GH3 b-glicosidase from cow rumen metagenome in complexed with xylose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-25 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.591, 119.324, 157.199 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 95.040 - 2.080 |
| R-factor | 0.17576 |
| Rwork | 0.174 |
| R-free | 0.21254 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5k6l |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.258 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 95.040 | 2.140 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Rmerge | 0.087 | 0.540 |
| Number of reflections | 58135 | |
| <I/σ(I)> | 10.9 | 3 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.6 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 2M (NH4)2SO4, 0.1M Bis-Tris pH 5.5, then soaked in 50mM xylose |
