5K6F
Crystal structure of prefusion-stabilized RSV F single-chain 9-19 DS-Cav1 variant.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-01-04 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | P 41 3 2 |
Unit cell lengths | 168.934, 168.934, 168.934 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.150 - 2.590 |
R-factor | 0.2043 |
Rwork | 0.203 |
R-free | 0.23740 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.458 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.854 | 2.640 |
High resolution limit [Å] | 2.550 | 2.550 |
Number of reflections | 26218 | |
<I/σ(I)> | 16.2 | 1.3 |
Completeness [%] | 100.0 | |
Redundancy | 19.9 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Na citrate pH 5.6, 15 % iso-propanol, 17 % PEG 4,000 |