5K5T
Crystal structure of the inactive form of human calcium-sensing receptor extracellular domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-11-15 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9792 |
Spacegroup name | F 2 2 2 |
Unit cell lengths | 126.330, 150.150, 214.580 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 107.210 - 3.100 |
R-factor | 0.223 |
Rwork | 0.222 |
R-free | 0.23860 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.150 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 107.210 | 3.600 |
High resolution limit [Å] | 3.100 | 3.100 |
<I/σ(I)> | 2.1 | |
Completeness [%] | 100 | |
Redundancy | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.5 M Li2SO4, 100 mM Tris pH 8.5, 2 mM CaCl2 |