Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-12-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 154.650, 56.920, 103.800 |
Unit cell angles | 90.00, 112.30, 90.00 |
Refinement procedure
Resolution | 48.018 - 2.649 |
R-factor | 0.2615 |
Rwork | 0.260 |
R-free | 0.27950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4rs8 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.753 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 48.018 |
High resolution limit [Å] | 2.649 |
Number of reflections | 22831 |
<I/σ(I)> | 8.5 |
Completeness [%] | 92.6 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 298 | 10% PEG 3000, 0.1 M sodium phosphate/citrate pH 6.2 |