5K5O
Structure of AspA-26mer DNA complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-12-20 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 32 1 2 |
Unit cell lengths | 96.627, 96.627, 187.093 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.005 - 3.200 |
R-factor | 0.2602 |
Rwork | 0.257 |
R-free | 0.28940 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4rs8 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.473 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.005 | 3.320 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.145 | 0.550 |
Number of reflections | 14191 | |
<I/σ(I)> | 6.5 | 1.1 |
Completeness [%] | 84.6 | |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 1 M citrate, 0.1 M cacodylate |