Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-19 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 102.400, 62.000, 62.000 |
Unit cell angles | 90.00, 109.70, 90.00 |
Refinement procedure
Resolution | 58.371 - 2.825 |
R-factor | 0.2363 |
Rwork | 0.232 |
R-free | 0.27620 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.532 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.371 | |
High resolution limit [Å] | 2.825 | 2.825 |
Rmerge | 0.099 | 0.490 |
Number of reflections | 17036 | |
<I/σ(I)> | 9 | 1.9 |
Completeness [%] | 99.1 | |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG 3350, citrate |