5K55
Human muscle fructose-1,6-bisphosphatase E69Q mutant in active R-state in complex with fructose-6-phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-01-22 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.9184 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 72.317, 72.317, 231.070 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.029 - 1.977 |
R-factor | 0.1876 |
Rwork | 0.185 |
R-free | 0.23490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5et5 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.621 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.030 | 2.100 |
High resolution limit [Å] | 1.977 | 1.977 |
Rmerge | 0.076 | 0.761 |
Number of reflections | 22004 | |
<I/σ(I)> | 22.79 | 3.15 |
Completeness [%] | 99.9 | 99.5 |
Redundancy | 9.56 | 9.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 292 | 10mM magnesium chloride, 2M sodium chloride, 10% PEG6000, 10mM Tris |