5K35
Structure of the Legionella effector, AnkB, in complex with human Skp1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-11-21 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9770 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.576, 57.039, 150.891 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.850 |
R-factor | 0.22203 |
Rwork | 0.219 |
R-free | 0.27473 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.253 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXDE |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.850 | 2.850 |
Rmerge | 0.622 | |
Number of reflections | 10735 | |
<I/σ(I)> | 48.5 | 6.42 |
Completeness [%] | 100.0 | 100 |
Redundancy | 14.2 | 14.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M trimethylamine N-oxide, 0.1 M Tris pH 8.5, 20% (w/v) PEG 2000 MME |