5K2G
Structure of GNNQQNY from yeast prion Sup35 in space group P21 determined by MicroED
Experimental procedure
Source type | ELECTRON MICROSCOPE |
Source details | TECNAI F20 TEM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-02-03 |
Detector | TVIPS F416 CMOS CAMERA |
Wavelength(s) | 0.0251 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 22.870, 4.930, 24.180 |
Unit cell angles | 90.00, 107.77, 90.00 |
Refinement procedure
Resolution | 23.030 - 1.100 |
R-factor | 0.1902 |
Rwork | 0.187 |
R-free | 0.22420 |
RMSD bond length | 0.019 |
RMSD bond angle | 1.337 |
Data scaling software | SCALEPACK |
Refinement software | REFMAC |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH | 7 | 273 | water |