5K2E
Structure of NNQQNY from yeast prion Sup35 with zinc acetate determined by MicroED
Experimental procedure
| Source type | ELECTRON MICROSCOPE |
| Source details | TECNAI F20 TEM |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2016-02-03 |
| Detector | TVIPS F416 CMOS CAMERA |
| Wavelength(s) | 0.0251 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 21.480, 4.950, 23.870 |
| Unit cell angles | 90.00, 103.95, 90.00 |
Refinement procedure
| Resolution | 23.170 - 1.000 |
| R-factor | 0.1564 |
| Rwork | 0.152 |
| R-free | 0.19430 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.525 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 90.000 | 90.000 | 1.080 |
| High resolution limit [Å] | 1.000 | 1.710 | 1.000 |
| Rmerge | 0.211 | 0.154 | 0.362 |
| Total number of observations | 13199 | ||
| Number of reflections | 2712 | ||
| <I/σ(I)> | 4.1 | ||
| Completeness [%] | 85.8 | 85.8 | 79.9 |
| Redundancy | 4.9 | 5.3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 273 | 1.0 M sodium acetate, 0.1 M HEPES, pH 7.0, 0.01 M zinc sulfate |
