5K22
Crystal structure of the complex between human PRL-2 phosphatase in reduced state and Bateman domain of human CNNM3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-12 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.6307 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 54.447, 126.717, 152.564 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.000 |
| R-factor | 0.23366 |
| Rwork | 0.231 |
| R-free | 0.27431 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.794 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.090 | 0.674 |
| Number of reflections | 10215 | |
| <I/σ(I)> | 38.9 | 3.1 |
| Completeness [%] | 98.9 | 93.88 |
| Redundancy | 9.7 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.8 M succinic acid, 30% w/v D-(+)-glucose monohydrate |
