5K1C
Crystal structure of the UAF1/WDR20/USP12 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 217.903, 217.903, 223.819 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.010 - 3.000 |
| R-factor | 0.23239 |
| Rwork | 0.230 |
| R-free | 0.27083 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5k16 5K19 and 5K1A |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.737 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.010 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.105 | |
| Number of reflections | 58534 | |
| <I/σ(I)> | 27.4 | 1.5 |
| Completeness [%] | 97.8 | 98.7 |
| Redundancy | 11.3 | 9.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 277 | 0.1 M MES, 1.3 M ammonium phosphate dibasic |
