5K0P
Crystal structure of the archaeosine synthase QueF-Like in the apo form
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97607 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.210, 124.995, 74.404 |
| Unit cell angles | 90.00, 112.27, 90.00 |
Refinement procedure
| Resolution | 69.000 - 1.940 |
| R-factor | 0.1589 |
| Rwork | 0.155 |
| R-free | 0.23850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jyx |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.247 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.000 | 1.980 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.049 | 0.685 |
| Number of reflections | 77458 | |
| <I/σ(I)> | 8.1 | 1.8 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 7.4 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 17-19% PEG3350, 0.15 M potassium thiocyanate, and 0.05% sodium azide. |
