5JZX
Crystal Structure of UDP-N-acetylenolpyruvoylglucosamine reductase (MurB) from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-09-03 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 274.924, 79.966, 138.996 |
| Unit cell angles | 90.00, 112.44, 90.00 |
Refinement procedure
| Resolution | 38.427 - 2.200 |
| R-factor | 0.21 |
| Rwork | 0.207 |
| R-free | 0.25939 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uxy |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.938 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.220 |
| High resolution limit [Å] | 2.170 | 2.170 |
| Number of reflections | 192931 | |
| <I/σ(I)> | 2.13 | |
| Completeness [%] | 98.8 | |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | 0.8M sodium formate, 15% PEG 4000, 0.2M sodium acetate |
