5JZV
The structure of D77G hCINAP-ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 3W1A |
| Synchrotron site | BSRF |
| Beamline | 3W1A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-15 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 61 |
| Unit cell lengths | 101.757, 101.757, 58.854 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.060 - 2.070 |
| R-factor | 0.192 |
| Rwork | 0.190 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3iil |
| RMSD bond length | 0.033 |
| RMSD bond angle | 2.023 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Number of reflections | 21343 | |
| <I/σ(I)> | 50.2 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 22.9 | 21.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 0.01M MAGNESIUM CHLORIDE HEXAHYDRATE, 0.05M MES MONOHYDRATE PH 5.6, 1.8M LITHIUM SULFATE MONOHYDRATE, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K |
