5JZN
Crystal structure of DCLK1-KD in complex with NVP-TAE684
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 61 |
| Unit cell lengths | 106.360, 106.360, 157.370 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 53.180 - 2.850 |
| R-factor | 0.1831 |
| Rwork | 0.181 |
| R-free | 0.22480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jzj |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.455 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.5.8) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 59.830 | 59.830 | 3.000 |
| High resolution limit [Å] | 2.850 | 9.010 | 2.850 |
| Rmerge | 0.195 | 0.098 | 0.845 |
| Rmeas | 0.216 | 0.108 | 0.934 |
| Rpim | 0.092 | 0.046 | 0.396 |
| Total number of observations | 126565 | 4242 | 18707 |
| Number of reflections | 23585 | ||
| <I/σ(I)> | 7.3 | 15.5 | 2.3 |
| Completeness [%] | 100.0 | 99.7 | 100 |
| Redundancy | 5.4 | 5.5 | 5.5 |
| CC(1/2) | 0.982 | 0.990 | 0.551 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7.5 | 298 | PEG400, Hepes |
