5JZM
Structure of wild type Amidase from Vibrio cholerae 0395 at low temparature at 1.8 Angstroms resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-11-30 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.920, 79.720, 133.580 |
| Unit cell angles | 90.00, 95.04, 90.00 |
Refinement procedure
| Resolution | 36.313 - 1.870 |
| R-factor | 0.1589 |
| Rwork | 0.158 |
| R-free | 0.19600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n57 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.352 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.313 | 1.960 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.086 | |
| Number of reflections | 118593 | |
| <I/σ(I)> | 1.34 | |
| Completeness [%] | 93.0 | |
| Redundancy | 1.79 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293.15 | 5% PEG 6000, 0.1M Na-citrate, 8% MPD |
