5JZK

The Structure of Ultra Stable Green Fluorescent Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	H 3 2
Unit cell lengths	137.182, 137.182, 147.647
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.220 - 1.900
R-factor	0.14405
Rwork	0.142
R-free	0.17639
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b3p
RMSD bond length	0.013
RMSD bond angle	1.572
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.220	1.940
High resolution limit [Å]	1.900	1.900
Rmerge	0.089	1.059
Number of reflections	41787
<I/σ(I)>	17.8	2.7
Completeness [%]	99.4	98.7
Redundancy	11.2	11.4
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		281	20 % w/v PEG 3350, 0.1 M Bis-Tris propane pH 6.5, 0.2 M Sodium nitrate