5JZJ
Crystal structure of DCLK1-KD in complex with AMPPN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-12-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.390, 81.270, 59.610 |
| Unit cell angles | 90.00, 92.10, 90.00 |
Refinement procedure
| Resolution | 56.350 - 1.710 |
| R-factor | 0.1784 |
| Rwork | 0.177 |
| R-free | 0.20830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y7j |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.000 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.2.7) |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT (2.10.0) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 56.350 | 56.350 | 1.740 |
| High resolution limit [Å] | 1.710 | 9.060 | 1.710 |
| Rmerge | 0.119 | 0.089 | 0.696 |
| Rmeas | 0.132 | 0.099 | 0.783 |
| Rpim | 0.058 | 0.043 | 0.351 |
| Total number of observations | 297540 | 2070 | 14795 |
| Number of reflections | 57833 | ||
| <I/σ(I)> | 9.4 | 17.6 | 3 |
| Completeness [%] | 99.9 | 99.5 | 99.8 |
| Redundancy | 5.1 | 5 | 4.9 |
| CC(1/2) | 0.991 | 0.983 | 0.777 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6.5 | 298 | PEG MME 5000, ammonium sulphate |
