5JYS
Pry1 CAP domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-11-25 |
| Detector | RIGAKU |
| Wavelength(s) | 1.514 |
| Spacegroup name | I 41 |
| Unit cell lengths | 69.105, 69.105, 97.730 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.553 - 1.901 |
| R-factor | 0.1985 |
| Rwork | 0.198 |
| R-free | 0.20830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ete |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.533 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASES |
| Refinement software | PHENIX ((dev_2405)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.553 | 1.990 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.082 | 0.428 |
| Number of reflections | 18355 | |
| <I/σ(I)> | 9.4 | 2.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.6 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 100mM Tris pH 8.5, 0.2M Magnesium chloride |
