5JYL
Human P-cadherin MEC1 with scFv TSP7 bound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-06-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 66.570, 49.410, 111.660 |
Unit cell angles | 90.00, 101.89, 90.00 |
Refinement procedure
Resolution | 109.260 - 2.550 |
R-factor | 0.21693 |
Rwork | 0.214 |
R-free | 0.27090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qok |
RMSD bond length | 0.008 |
RMSD bond angle | 1.291 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 109.260 | 2.690 |
High resolution limit [Å] | 2.550 | 2.550 |
Rmerge | 0.125 | 0.670 |
Number of reflections | 23310 | |
<I/σ(I)> | 10.4 | 2.4 |
Completeness [%] | 99.1 | 96.3 |
Redundancy | 5.4 | 5.2 |
CC(1/2) | 0.994 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 200 mM potassium phosphate monobasic, 20% PEG 3350 |