5JYI

Trypsin bound with succinic acid at 1.9A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-08-14
Detector	MARRESEARCH
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	46.320, 53.180, 76.810
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.405 - 1.914
R-factor	0.1576
Rwork	0.153
R-free	0.20140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4i8h
RMSD bond length	0.008
RMSD bond angle	1.164
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall
Low resolution limit [Å]	39.660
High resolution limit [Å]	1.900
Number of reflections	15134
<I/σ(I)>	19
Completeness [%]	99.3
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	5.5	298.15	0.1M Succinic acid,0.1M Sodium Acetate ph 5.8,15%(w/v) PEG3350