5JYC
Crystal structure of the E153Q mutant of the CFTR inhibitory factor Cif containing the adducted 14,15-EET hydrolysis intermediate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2011-04-19 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 168.930, 83.602, 89.241 |
| Unit cell angles | 90.00, 100.38, 90.00 |
Refinement procedure
| Resolution | 35.410 - 2.000 |
| R-factor | 0.1549 |
| Rwork | 0.153 |
| R-free | 0.19100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kd2 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.829 |
| Data reduction software | XDS (Dec 6th, 2010) |
| Data scaling software | XDS (Dec 6th, 2010) |
| Phasing software | PHENIX ((1.7_650: ???)) |
| Refinement software | PHENIX ((1.10_2155: 000)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.410 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.106 | 0.450 |
| Number of reflections | 82560 | |
| <I/σ(I)> | 14.27 | 4.44 |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 6.24 | 6.16 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | PEG 8K, calcium chloride, sodium acetate |
