5JXX
Crystal structure of UDP-N-acetylglucosamine O-acyltransferase (LpxA) from Moraxella catarrhalis RH4.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR-H |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-05-20 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.611, 136.897, 90.585 |
| Unit cell angles | 90.00, 91.64, 90.00 |
Refinement procedure
| Resolution | 45.390 - 3.001 |
| R-factor | 0.238 |
| Rwork | 0.235 |
| R-free | 0.29290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4e6u |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.578 |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.390 | 3.108 |
| High resolution limit [Å] | 3.000 | 3.001 |
| Number of reflections | 30503 | |
| <I/σ(I)> | 4.76 | |
| Completeness [%] | 80.0 | |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 0.05 M Citric Bis-Tris, Propane, pH 5.0, 16% (w/v) Polyethylene glycol 3350 |
