5JX4
Crystal structure of E36-G37del mutant of the Bacillus caldolyticus cold shock protein.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2015-08-21 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 34.009, 44.077, 36.484 |
Unit cell angles | 90.00, 99.28, 90.00 |
Refinement procedure
Resolution | 18.797 - 1.800 |
R-factor | 0.1515 |
Rwork | 0.150 |
R-free | 0.18210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hax |
RMSD bond length | 0.013 |
RMSD bond angle | 1.335 |
Data reduction software | XDS |
Data scaling software | Aimless (0.3.11) |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 18.800 | 1.840 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.048 | 0.114 |
Number of reflections | 9547 | |
<I/σ(I)> | 23.6 | |
Completeness [%] | 95.6 | 70.3 |
Redundancy | 3.5 | 2.5 |
CC(1/2) | 0.998 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291 | 0.1 M Sodium Acetate, Poly(ethylene glycol) methyl ether 2000 30%, 0.2 M Ammonium Sulfate. Protein 20 mg/mL in 20 mM Sodium phosphate. |