5JWZ
Structure of a Putative Xyloglucanase from the Cellulolytic Bacteria Streptomyces sp. SirexAA-E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-22 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97857 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 104.149, 140.181, 116.711 |
| Unit cell angles | 90.00, 113.92, 90.00 |
Refinement procedure
| Resolution | 29.887 - 1.695 |
| R-factor | 0.1281 |
| Rwork | 0.128 |
| R-free | 0.16880 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.127 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.738 |
| High resolution limit [Å] | 1.695 | 1.695 |
| Rmerge | 0.102 | 0.781 |
| Number of reflections | 159374 | |
| <I/σ(I)> | 24.67 | 3.1 |
| Completeness [%] | 94.4 | 70 |
| Redundancy | 13 | 12.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 20% PEG 6K, 200mM Calcium Chloride, and 100mM of HEPES buffered at pH 7.0 |
