5JWQ
Crystal structure of KaiC S431E in complex with foldswitch-stabilized KaiB from Thermosynechococcus elongatus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-03-16 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 155.920, 155.920, 155.920 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 90.020 - 3.871 |
| R-factor | 0.3283 |
| Rwork | 0.327 |
| R-free | 0.34520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.498 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.020 | 4.080 |
| High resolution limit [Å] | 3.870 | 3.870 |
| Rmerge | 0.272 | 1.050 |
| <I/σ(I)> | 7.2 | 2.5 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 8.4 | 8.3 |
| CC(1/2) | 0.987 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.75 | 295 | 0.1M Sodium citrate pH 5.75 and 22% PEG3000 |
