5JW9
The Crystal Structure of ELL2 Oclludin Domain and AFF4 peptide
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-01-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9700 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.641, 57.422, 61.338 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.920 - 2.003 |
| R-factor | 0.1991 |
| Rwork | 0.197 |
| R-free | 0.24830 |
| Structure solution method | MAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.569 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.142 | 0.079 | |
| Number of reflections | 13052 | ||
| <I/σ(I)> | 6.5 | ||
| Completeness [%] | 100.0 | 99.9 | 100 |
| Redundancy | 14 | 13.2 | 12.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 0.2M NaCl, 10mM MgCl, 0.3M Na3 Citrate, 0.2M Na Thiocyanate, 0.1M Hepes 7.4 |
