5JVU
The neck-linker and alpha 7 helix of Drosophila melanogaster kinesin-1 fused to EB1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-12-07 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97934 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.973, 37.783, 52.706 |
Unit cell angles | 90.00, 114.96, 90.00 |
Refinement procedure
Resolution | 28.263 - 1.948 |
R-factor | 0.2001 |
Rwork | 0.198 |
R-free | 0.24300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yib |
RMSD bond length | 0.007 |
RMSD bond angle | 0.793 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.263 | 2.072 |
High resolution limit [Å] | 1.948 | 1.948 |
Rmerge | 0.083 | 0.589 |
Number of reflections | 12095 | |
<I/σ(I)> | 16.6 | 2.1 |
Completeness [%] | 99.0 | 97.3 |
Redundancy | 4.8 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 20% (w/v) methoxy polyethylene glycol (MEPEG) 5000, 100 mM Li2SO4, 100 mM MES/Acetate pH 5.5, 10 mM gamma-caprolactone |