5JVP
The neck-linker and alpha 7 helix of Homo sapiens CENP-E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-08-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 100.609, 100.609, 107.627 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.785 - 2.100 |
| R-factor | 0.239 |
| Rwork | 0.237 |
| R-free | 0.27390 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.427 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.786 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.114 | 0.360 |
| Number of reflections | 71015 | |
| <I/σ(I)> | 16.6 | 12.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 16.6 | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 18% (w/v) MEPEG 2000, 175 mM Li2SO4, 100 mM MES pH 6.0, 1 mM CdCl2 |
