5JVK
Structural insights into a family 39 glycoside hydrolase from the gut symbiont Bacteroides cellulosilyticus WH2.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-09-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.8726, 1.2543 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 157.540, 157.540, 155.644 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.770 - 2.500 |
| R-factor | 0.20711 |
| Rwork | 0.206 |
| R-free | 0.24117 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.112 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.900 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.075 | 0.446 |
| Number of reflections | 71297 | |
| <I/σ(I)> | 14.5 | 14.5 |
| Completeness [%] | 92.0 | 65.7 |
| Redundancy | 3.5 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2 M AmSO4, 0.1 M CAPS pH 8 and 0.2 M LiSO4 |
