5JVH
The crystal structure large ribosomal subunit (50S) of Deinococcus radiodurans in complex with evernimicin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.033 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 169.475, 407.380, 692.552 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.845 - 3.580 |
| R-factor | 0.2059 |
| Rwork | 0.204 |
| R-free | 0.24710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zjr |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.299 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 3.640 |
| High resolution limit [Å] | 3.580 | 9.620 | 3.580 |
| Rmerge | 0.121 | 0.037 | 0.840 |
| Total number of observations | 982811 | ||
| Number of reflections | 264969 | ||
| <I/σ(I)> | 7.4 | ||
| Completeness [%] | 94.4 | 88.1 | 93.5 |
| Redundancy | 3.7 | 4.1 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | Magnesium chloride, HEPES, ammonium chloride, ethanol, 2-ethyl-1,3-hexandiol |
