5JVH
The crystal structure large ribosomal subunit (50S) of Deinococcus radiodurans in complex with evernimicin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-04-12 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.033 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 169.475, 407.380, 692.552 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.845 - 3.580 |
R-factor | 0.2059 |
Rwork | 0.204 |
R-free | 0.24710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2zjr |
RMSD bond length | 0.008 |
RMSD bond angle | 1.299 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 3.640 |
High resolution limit [Å] | 3.580 | 9.620 | 3.580 |
Rmerge | 0.121 | 0.037 | 0.840 |
Total number of observations | 982811 | ||
Number of reflections | 264969 | ||
<I/σ(I)> | 7.4 | ||
Completeness [%] | 94.4 | 88.1 | 93.5 |
Redundancy | 3.7 | 4.1 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | Magnesium chloride, HEPES, ammonium chloride, ethanol, 2-ethyl-1,3-hexandiol |