5JVF
Crystal Structure of Apo-FleN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-04 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.95372 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 66.250, 66.250, 112.870 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.573 - 1.660 |
| R-factor | 0.1901 |
| Rwork | 0.189 |
| R-free | 0.21710 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.095 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.690 | 1.750 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Number of reflections | 65124 | |
| <I/σ(I)> | 17.6 | |
| Completeness [%] | 99.6 | |
| Redundancy | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.2M Ammonium iodide, 20% PEG3350 |
