5JTV
USP7CD-UBL45 in complex with Ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2014-10-29 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 113.611, 115.520, 257.822 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.954 - 3.312 |
| R-factor | 0.2402 |
| Rwork | 0.239 |
| R-free | 0.26850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nbf |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.808 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_2210) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.954 | 115.450 | 3.284 |
| High resolution limit [Å] | 3.273 | 15.182 | 3.273 |
| Rmerge | 0.127 | 0.036 | 0.853 |
| Rmeas | 0.139 | ||
| Rpim | 0.055 | ||
| Total number of observations | 323509 | ||
| Number of reflections | 52986 | ||
| <I/σ(I)> | 11.4 | ||
| Completeness [%] | 100.0 | 98.5 | 99.6 |
| Redundancy | 6.1 | 5 | 6.3 |
| CC(1/2) | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.2 M K chloride, 0.05 M HEPES pH 7.5 and 35% v/v Pentaerythritol propoxylate |
