5JTI
Crystal structure of the human Tankyrase 1 (TNKS) SAM domain (D1055R), crystal form 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.934, 55.480, 79.414 |
| Unit cell angles | 90.00, 102.90, 90.00 |
Refinement procedure
| Resolution | 77.410 - 2.900 |
| R-factor | 0.195 |
| Rwork | 0.193 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jrt |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.190 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.410 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.229 | |
| Number of reflections | 15085 | |
| <I/σ(I)> | 7.4 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 285.15 | 0.1 M Bis-Tris pH 5.5 0.2 M Magnesium Chloride 25% PEG 3350 |
