5JTH
Crystal structure of E67A calmodulin - CaM:RM20 analog complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-12 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 29.094, 57.053, 44.871 |
| Unit cell angles | 90.00, 97.18, 90.00 |
Refinement procedure
| Resolution | 28.000 - 1.840 |
| R-factor | 0.15877 |
| Rwork | 0.157 |
| R-free | 0.19640 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.759 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 1.840 |
| Number of reflections | 12634 |
| <I/σ(I)> | 31.8 |
| Completeness [%] | 99.8 |
| Redundancy | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.1M Sodium Acetate pH 4.6, 25%(w/v) PEG 4000, 20mM CaCl2 |
