5JTF

Crystal structure of ArsN N-acetyltransferase from Pseudomonas putida KT2440

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-08-05
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0000
Spacegroup name	P 43 21 2
Unit cell lengths	67.017, 67.017, 206.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.644 - 2.156
R-factor	0.2391
Rwork	0.238
R-free	0.26550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yvo
RMSD bond length	0.004
RMSD bond angle	0.905
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.644	2.200
High resolution limit [Å]	2.156	2.160
Number of reflections	25849
<I/σ(I)>	6.88	2.91
Completeness [%]	97.8	90.81
Redundancy	5.16	4.31

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.2 M Sodium acetate, 0.1 M TRIS HC (pH 8.5), 30% PEG 4000