5JST

MBP fused MDV1 coiled coil

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2015-05-30
Detector	ADSC QUANTUM 315
Wavelength(s)	1.25530
Spacegroup name	C 1 2 1
Unit cell lengths	128.515, 102.942, 79.267
Unit cell angles	90.00, 102.51, 90.00

Refinement procedure

Resolution	36.846 - 2.199
R-factor	0.2268
Rwork	0.225
R-free	0.27360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3dm0 2xu6
RMSD bond length	0.007
RMSD bond angle	0.879
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.240
High resolution limit [Å]	2.199	5.970	2.200
Rmerge	0.063	0.036	0.697
Rmeas	0.072	0.041	0.801
Rpim	0.036	0.021	0.393
Total number of observations	205521
Number of reflections	50774
<I/σ(I)>	15.4
Completeness [%]	99.2	87.1	100
Redundancy	4	3.8	4.1
CC(1/2)		0.997	0.696

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	1.3~1.7 M Sodium formate, 25% PEG 3350, 0.1 M CaCl2, 0.1 M Sodium acetate/Acetic acid, pH 4.5