5JSI
Structure of membrane protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-11-10 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.85 |
Spacegroup name | P 1 |
Unit cell lengths | 40.793, 56.771, 57.286 |
Unit cell angles | 63.61, 78.48, 80.21 |
Refinement procedure
Resolution | 50.800 - 2.000 |
R-factor | 0.17938 |
Rwork | 0.177 |
R-free | 0.21999 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.751 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | SHELXCD |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.000 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.051 | 0.501 |
Number of reflections | 49905 | |
<I/σ(I)> | 8.64 | 1.43 |
Completeness [%] | 81.9 | 68.6 |
Redundancy | 1.88 | 1.83 |
CC(1/2) | 0.997 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293 | in meso lipidic cubic phase crystallization |