5JS1
Human Argonaute2 Bound to an siRNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2014-12-17 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.979500 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.932, 107.119, 68.198 |
| Unit cell angles | 90.00, 106.98, 90.00 |
Refinement procedure
| Resolution | 31.531 - 2.499 |
| R-factor | 0.2181 |
| Rwork | 0.216 |
| R-free | 0.24750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ola |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.288 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.830 | 2.600 |
| High resolution limit [Å] | 2.499 | 2.500 |
| Rmerge | 0.049 | 0.438 |
| Number of reflections | 29397 | |
| <I/σ(I)> | 17.2 | |
| Completeness [%] | 98.1 | |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 16% PEG 3350, 50mM Phenol, 10% Isopropanol, 5mM Magnesium Chloride, 100mM Tris |
