5JRT
Crystal structure of the human Tankyrase 2 (TNKS2) SAM domain (DH902/924RE)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 65 |
| Unit cell lengths | 56.630, 56.630, 46.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.320 - 1.530 |
| R-factor | 0.203 |
| Rwork | 0.201 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1v85_A 3bs5_B 3bq_7 2e8o_A 2gle_A |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.940 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.050 | 1.580 |
| High resolution limit [Å] | 1.530 | 1.530 |
| Rmerge | 0.058 | |
| Number of reflections | 12770 | |
| <I/σ(I)> | 17 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 17 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 285.15 | PEG 3350 20% 0.1 MTris-HCl pH 8.5 0.2 M Ammonium Acetate |
