5JRB
Rad52(1-212) K102A/K133A/E202A mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-12-15 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9800 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 110.955, 98.516, 116.367 |
Unit cell angles | 90.00, 91.99, 90.00 |
Refinement procedure
Resolution | 49.258 - 2.405 |
R-factor | 0.2261 |
Rwork | 0.224 |
R-free | 0.27310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1kn0 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.018 |
Data reduction software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.300 | 2.550 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.095 | 0.633 |
Number of reflections | 190708 | |
<I/σ(I)> | 9.2 | 1.9 |
Completeness [%] | 99.6 | 99 |
Redundancy | 3.5 | 3.5 |
CC(1/2) | 0.995 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.05M Sodium malonate pH6.0, 5% PEG 3350 |