5JR5
RHCC in Complex with Elemental Sulfur
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-07-04 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54192 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 110.520, 110.520, 71.040 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.751 - 1.900 |
| R-factor | 0.185 |
| Rwork | 0.182 |
| R-free | 0.20660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fe6 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.107 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.751 |
| High resolution limit [Å] | 1.900 |
| Number of reflections | 39292 |
| <I/σ(I)> | 6.2 |
| Completeness [%] | 98.9 |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 1.6M ammonium sulfate and 100 mM Tris pH 8.0 |
