5JQZ

Designed two-ring homotetramer at 3.8A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-05-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 21 21 2
Unit cell lengths	70.690, 37.331, 58.716
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	58.716 - 3.830
R-factor	0.433
Rwork	0.428
R-free	0.47850
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.681
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (dev_1616)

Data quality characteristics

	Overall
Low resolution limit [Å]	58.720
High resolution limit [Å]	3.830
Number of reflections	1573
<I/σ(I)>	90.7
Completeness [%]	93.9
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.2 M Sodium Acetate trihydrate, 0.1 M Tris hydrochloride pH 8.5 and 30 % PEG 4,000