5JQW
The crystal structure of phosphoribosylaminoimidazole carboxylase ATPase subunit of Francisella tularensis subsp. tularensis SCHU S4 in complex with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97938 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 85.230, 85.230, 103.992 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.369 - 2.060 |
| R-factor | 0.1983 |
| Rwork | 0.197 |
| R-free | 0.22870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mam |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.506 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((dev-2386_1692: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.370 | 2.100 |
| High resolution limit [Å] | 2.060 | 2.060 |
| Rmerge | 0.090 | 0.691 |
| Number of reflections | 24555 | |
| <I/σ(I)> | 36.8 | 2.8 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 8.1 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.8M LiCl, 0.1M Tris, 10mM ADP, 32% (w/v) PEG4000 |
