5JQP
Crystal structure of ER glucosidase II heterodimeric complex consisting of catalytic subunit and the binding domain of regulatory subunit
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 95 |
Detector technology | PIXEL |
Collection date | 2015-12-15 |
Detector | DECTRIS EIGER R 4M |
Wavelength(s) | 1.10000 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 82.716, 88.712, 173.103 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.200 |
R-factor | 0.155 |
Rwork | 0.153 |
R-free | 0.20600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5dkx |
RMSD bond length | 0.015 |
RMSD bond angle | 1.596 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.108 | 0.499 |
Number of reflections | 64671 | |
<I/σ(I)> | 11.8 | 2.2 |
Completeness [%] | 99.4 | 100 |
Redundancy | 4.4 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 20% PEG 3350, 100 mM Bis-Tris (pH 6.5), 0.2 M ammonium phosphate |