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5JQP

Crystal structure of ER glucosidase II heterodimeric complex consisting of catalytic subunit and the binding domain of regulatory subunit

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsPHOTON FACTORY BEAMLINE BL-1A
Synchrotron sitePhoton Factory
BeamlineBL-1A
Temperature [K]95
Detector technologyPIXEL
Collection date2015-12-15
DetectorDECTRIS EIGER R 4M
Wavelength(s)1.10000
Spacegroup nameP 21 2 21
Unit cell lengths82.716, 88.712, 173.103
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution20.000 - 2.200
R-factor0.155
Rwork0.153
R-free0.20600
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)5dkx
RMSD bond length0.015
RMSD bond angle1.596
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareMOLREP
Refinement softwareREFMAC (5.8.0069)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.240
High resolution limit [Å]2.2002.200
Rmerge0.1080.499
Number of reflections64671
<I/σ(I)>11.82.2
Completeness [%]99.4100
Redundancy4.44.6
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP6.529320% PEG 3350, 100 mM Bis-Tris (pH 6.5), 0.2 M ammonium phosphate

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