5JQL
Crystal Structure of Phosphatidic acid Transporter Ups1/Mdm35 Void of Bound Phospholipid from Saccharomyces Cerevisiae at 2.9 Angstroms Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.311, 130.736, 84.271 |
| Unit cell angles | 90.00, 103.09, 90.00 |
Refinement procedure
| Resolution | 48.404 - 2.900 |
| R-factor | 0.2025 |
| Rwork | 0.200 |
| R-free | 0.24770 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.310 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.8_1069) |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.950 |
| High resolution limit [Å] | 2.900 | 7.860 | 2.900 |
| Rmerge | 0.074 | 0.043 | 0.438 |
| Rmeas | 0.087 | ||
| Rpim | 0.045 | ||
| Total number of observations | 279505 | ||
| Number of reflections | 76995 | ||
| <I/σ(I)> | 14.9 | ||
| Completeness [%] | 98.4 | 94 | 97 |
| Redundancy | 3.6 | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 289 | 8% Tacsimate, pH5.0, 20% PEG 3350, 200mM Sodium thiocyanate |
